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lsab system kit  (Agilent technologies)


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    Structured Review

    Agilent technologies lsab system kit
    Lsab System Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 25395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lsab+kit/Streptavidin/pmc13649940-61-24-27
    Average 99 stars, based on 25395 article reviews
    lsab system kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: The Effect of Low-Intensity Pulsed Ultrasound on Bone Regeneration and the Expression of Osterix and Cyclooxygenase-2 during Critical-Size Bone Defect Repair.
    Article Snippet: Afterwards, rabbit polyclonal anti-Sp7/Osterix antibody (ab 22552 Abcam, Abcam, Cambridge, UK) and rabbit polyclonal anti-COX-2 antibody (PA5-27238, Invitrogen, Waltham, MA, USA) were used for overnight incubation with tissue samples. .. After antibody incubation, the specimens were then washed and incubated with a secondary biotinylated antibody for 45 min. For visualization, peroxidase-conjugated streptavidin (LSAB+ Kit, DakoCytomation, Glostrup, Denmark) and 3,30-diaminobenzidine Int. ..

    Article Title: Characterisation and Sensitivity of a Canine Mast Cell Tumour Line to Oncolytic Viruses
    Article Snippet: Cells were permeabilised using 0.1% Triton X‐100 (Sigma‐Aldrich, Oakville, ON, Canada) for 5 min, rinsed with wash buffer (Dako, Carpinteria, CA, USA), blocked with protein‐free blocking solution for 10 min (Dako), followed by incubation with primary rabbit antibodies specific for KIT (Dako; 1:100; phospho‐KIT [p‐KIT] and VEGFR2 [all from Cell Signalling Technology; used at 1:100]) for 1 h at room temperature. .. A LSAB Kit (Streptavidin‐biotin‐HRP Labelling Kit for Immunohistochemistry) (Dako, Carpinteria, CA, USA) was used as per the manufacturer's protocol using 30‐min incubation times for anti‐rabbit biotinylated antibodies and peroxidase‐labelled streptavidin. .. Reactions were visualised with 3′,3′‐diaminobenzidine (DAB; Dako, Carpinteria, CA, USA).

    Article Title: Characterisation and Sensitivity of a Canine Mast Cell Tumour Line to Oncolytic Viruses.
    Article Snippet: Cells were permeabilised using 0.1% Triton X- 100 (SigmaAldrich, Oakville, ON, Canada) for 5 min, rinsed with wash buffer (Dako, Carpinteria, CA, USA), blocked with proteinfree blocking solution for 10 min (Dako), followed by incubation with primary rabbit antibodies specific for KIT (Dako; 1:100; phospho- KIT [p- KIT] and VEGFR2 [all from Cell Signalling Technology; used at 1:100]) for 1 h at room temperature. .. A LSAB Kit (Streptavidin- biotin- HRP Labelling Kit for Immunohistochemistry) (Dako, Carpinteria, CA, USA) was used as per the manufacturer's protocol using 30- min incubation times for anti- rabbit biotinylated antibodies and peroxidase- labelled streptavidin. .. Reactions were visualised with 3′,3′- diaminobenzidine (DAB; Dako, Carpinteria, CA, USA).

    Article Title: Asian dust exacerbates cedar pollen-induced allergic conjunctivitis in mice.
    Article Snippet: Asian sand dust (ASD), a type of fine particulate matter originating from arid regions of East Asia, is known to exacerbate respiratory diseases, but its impact on the ocular surface remains poorly understood.. Given the increasing prevalence of allergic conjunctivitis in Japan, particularly during the Japanese cedar pollen (JCP) season, we investigated whether ASD exposure aggravates ocular allergic inflammation.. Using a mouse model sensitized with JCP, we found that conjunctival instillation of ASD in combination with JCP significantly increased clinical signs such as hyperemia and edema, along with elevated serum IgE levels and enhanced inflammatory cell infiltration compared to JCP alone.

    Immunohistochemistry:

    Article Title: Characterisation and Sensitivity of a Canine Mast Cell Tumour Line to Oncolytic Viruses
    Article Snippet: Cells were permeabilised using 0.1% Triton X‐100 (Sigma‐Aldrich, Oakville, ON, Canada) for 5 min, rinsed with wash buffer (Dako, Carpinteria, CA, USA), blocked with protein‐free blocking solution for 10 min (Dako), followed by incubation with primary rabbit antibodies specific for KIT (Dako; 1:100; phospho‐KIT [p‐KIT] and VEGFR2 [all from Cell Signalling Technology; used at 1:100]) for 1 h at room temperature. .. A LSAB Kit (Streptavidin‐biotin‐HRP Labelling Kit for Immunohistochemistry) (Dako, Carpinteria, CA, USA) was used as per the manufacturer's protocol using 30‐min incubation times for anti‐rabbit biotinylated antibodies and peroxidase‐labelled streptavidin. .. Reactions were visualised with 3′,3′‐diaminobenzidine (DAB; Dako, Carpinteria, CA, USA).

    Article Title: Mortalin promotes the evolution of androgen-independent prostate cancer through Wnt/β-catenin signaling pathway
    Article Snippet: .. Immunohistochemistry staining was performed on all tissues using the DAKO LSAB kit (DAKO A/S, Glostrup, Denmark). ..

    Article Title: Characterisation and Sensitivity of a Canine Mast Cell Tumour Line to Oncolytic Viruses.
    Article Snippet: Cells were permeabilised using 0.1% Triton X- 100 (SigmaAldrich, Oakville, ON, Canada) for 5 min, rinsed with wash buffer (Dako, Carpinteria, CA, USA), blocked with proteinfree blocking solution for 10 min (Dako), followed by incubation with primary rabbit antibodies specific for KIT (Dako; 1:100; phospho- KIT [p- KIT] and VEGFR2 [all from Cell Signalling Technology; used at 1:100]) for 1 h at room temperature. .. A LSAB Kit (Streptavidin- biotin- HRP Labelling Kit for Immunohistochemistry) (Dako, Carpinteria, CA, USA) was used as per the manufacturer's protocol using 30- min incubation times for anti- rabbit biotinylated antibodies and peroxidase- labelled streptavidin. .. Reactions were visualised with 3′,3′- diaminobenzidine (DAB; Dako, Carpinteria, CA, USA).

    Staining:

    Article Title: Mortalin promotes the evolution of androgen-independent prostate cancer through Wnt/β-catenin signaling pathway
    Article Snippet: .. Immunohistochemistry staining was performed on all tissues using the DAKO LSAB kit (DAKO A/S, Glostrup, Denmark). ..

    other:

    Article Title: Synergistic action of gemcitabine and celecoxib in promoting the antitumor efficacy of anti-programmed death-1 monoclonal antibody by triggering immunogenic cell death
    Article Snippet: Streptavidin-linked secondary antibody (LSAB+ kit, Dako, Agilent Technologies, Santa Clara, CA, USA) was applied for 30 min, which was followed by application streptavidin peroxidase for 15 min. After incubation for 5 min in the substrate developer, counterstaining with hematoxylin was conducted for 3 min.

    Article Title: Human NLRC4 expression promotes cancer survival and associates with type I interferon signaling and immune infiltration
    Article Snippet: We used antibodies against the following proteins: CD3 (clone 2GV6, prediluted, Roche Diagnostics), CD8 (clone C8/144B, diluted 1:25, Dako), CD68 (clone PG-M1, diluted 1:50, Dako), and CD163 (clone 10D6, diluted 1:100, Leica Novocastra Laboratories).



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